Simultaneous multiplex Polymerase Chain Reaction detection of Salmonella spp., Escherichia coli O157, Vibrio parahaemolyticus, Vibrio cholerae, Listeria monocytogenes and Campylobacter spp.
نویسندگان
چکیده
منابع مشابه
Prevalence of Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, and Salmonella spp. in seafood products using multiplex polymerase chain reaction.
Although several etiological agents can be transmitted through seafood consumption, Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, and Salmonella spp. are considered among the most important pathogens in terms of public health and disease. In this study, multiplex polymerase chain reaction (PCR), as a rapid and cost-effective method, was used to determine the prevalence...
متن کاملMultiplex Real-time Polymerase Chain Reaction Assays for Simultaneous Detection of Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus
OBJECTIVES A multiplex real-time polymerase chain reaction (RT-PCR) method was developed for the identification of three Vibrio species: Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus. METHODS Specific primers and probes targeting the hlyA, tlh, and vvhA genes were selected and used for multiplex real-time PCR to confirm the identification of V. cholerae, V. parahaemolyticus,...
متن کاملsimultaneous detection of escherichia coli o157:h7, toxigenic vibrio cholerae, and salmonella typhimurium by multiplex pcr
background escherichia coli o157:h7, vibrio cholerae, and salmonella typhimurium are pathogenic bacteria found in contaminated water and food. no assay method is currently available on simultaneous detection or identification of all the three pathogens. our aim was to develop a rapid and reliable method for this purpose. materials and methods a protocol for sample collection, and a pcr procedur...
متن کاملA multipathogen selective enrichment broth for simultaneous growth of Salmonella spp., Vibrio parahaemolyticus, and Vibrio cholerae.
A selective enrichment broth (SVV) was formulated to allow concurrent growth of Salmonella spp., V. parahaemolyticus, and V. cholerae. Potassium tellurite and sodium citrate were added as the inhibitors, while glucose, mannitol, anhydrous sodium sulfite and sodium pyruvate were employed as the growth-promoters. When mixed in equal or varied proportions, the target pathogens in SVV had a great a...
متن کاملSpecific detection of Salmonella spp. by multiplex polymerase chain reaction.
Three sets of oligonucleotide primers were used in the polymerase chain reaction (PCR) assay to detect Salmonella species. phoP primers specific to the phoP/phoQ loci of coliform pathogenic bacteria such as Salmonella, Shigella, Escherichia coli, and Citrobacter species served as presumptive indicators of enteric bacteria. In addition to the phoP primers, the Hin and the H-1i primers, which tar...
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ژورنال
عنوان ژورنال: Food Research
سال: 2018
ISSN: 2550-2166,2550-2166
DOI: 10.26656/fr.2017.2(3).002